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anti- avb6 antibody (clone 10d5)  (Millipore)


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    Structured Review

    Millipore anti- avb6 antibody (clone 10d5)
    Anti Avb6 Antibody (Clone 10d5), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-+avb6+antibody+(clone+10d5)/integrin+avb6+antibody/pm38519394-42-7-13
    Average 90 stars, based on 1 article reviews
    anti- avb6 antibody (clone 10d5) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: The Ability of Integrin α v β 3 To Function as a Receptor for Foot-and-Mouth Disease Virus Is Not Dependent on the Presence of Complete Subunit Cytoplasmic Domains
    Article Snippet: Thirty minutes prior to infection, paired transfected cell cultures were incubated at room temperature with the following function-blocking anti-integrin MAbs at a concentration of 25 mg/ml (all antibodies were from Chemicon International Inc): anti-avb3 (clone LM609; MAB1976), anti-avb5 (clone P1F6; MAB1961), anti-avb6 (clone 10D5; MAB2077Z), anti-a5 (clone CLB-705; MAB1986), and anti-b1 (clone 6S6; MAB2253).

    Article Title: Integrin-αvβ6, a Putative Receptor for Foot-and-Mouth Disease Virus, Is Constitutively Expressed in Ruminant Airways
    Article Snippet: Sections were then washed with staining buffer (pH 7.4, PBS supplemented with 4 mM MgCl2 and 0.5% Tween 80) (Sigma-Aldrich), blocked for 15 min with staining buffer 1 10% heat-inactivated normal sheep serum (NShS) and incubated for 1 hr with mouse IgG2a anti-integrin-avb6 (clone 10D5; Chemicon Europe, Chandlers Ford, UK) or control mouse IgG2a (clone OX34; Serotec, Kidlington, UK) diluted to 0.5 mg/ml in staining buffer 1 10% NShS.

    Article Title: Integrin-αvβ6 targeted peptide-toxin therapy in a novel αvβ6-expressing immunocompetent model of pancreatic cancer.
    Article Snippet: Cells were incubated with 1 ml of anti- avb6 antibody (clone 10D5) (MAB2077Z, Millipore) for 1 h at 4 C. The cells werewashed againwith cold DMEM0.1%/0.1% buffer and incubated with Alexa Fluor 488econjugated goat anti-mouse secondary antibody (10337882, ThermoFisher) antibody at a 1:200 dilution for 30 min at 4 C in the dark.

    Article Title: Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins.
    Article Snippet: Members of the av family of integrins regulate activation of transforming growth factor beta (TGFb) and are directly involved in pro-tumorigenic phenotypes.. Thus, av integrins may be therapeutic targets for fibrosis and cancer, yet the isolation of selective inhibitors is currently a challenge.. We generated synthetic antibodies selective for av integrins by phage display selections on cell lines that displayed integrin heterodimers.

    Incubation:

    Article Title: The Ability of Integrin α v β 3 To Function as a Receptor for Foot-and-Mouth Disease Virus Is Not Dependent on the Presence of Complete Subunit Cytoplasmic Domains
    Article Snippet: Thirty minutes prior to infection, paired transfected cell cultures were incubated at room temperature with the following function-blocking anti-integrin MAbs at a concentration of 25 mg/ml (all antibodies were from Chemicon International Inc): anti-avb3 (clone LM609; MAB1976), anti-avb5 (clone P1F6; MAB1961), anti-avb6 (clone 10D5; MAB2077Z), anti-a5 (clone CLB-705; MAB1986), and anti-b1 (clone 6S6; MAB2253).

    Article Title: Integrin-αvβ6, a Putative Receptor for Foot-and-Mouth Disease Virus, Is Constitutively Expressed in Ruminant Airways
    Article Snippet: Sections were then washed with staining buffer (pH 7.4, PBS supplemented with 4 mM MgCl2 and 0.5% Tween 80) (Sigma-Aldrich), blocked for 15 min with staining buffer 1 10% heat-inactivated normal sheep serum (NShS) and incubated for 1 hr with mouse IgG2a anti-integrin-avb6 (clone 10D5; Chemicon Europe, Chandlers Ford, UK) or control mouse IgG2a (clone OX34; Serotec, Kidlington, UK) diluted to 0.5 mg/ml in staining buffer 1 10% NShS.

    Article Title: Integrin-αvβ6 targeted peptide-toxin therapy in a novel αvβ6-expressing immunocompetent model of pancreatic cancer.
    Article Snippet: Cells were incubated with 1 ml of anti- avb6 antibody (clone 10D5) (MAB2077Z, Millipore) for 1 h at 4 C. The cells werewashed againwith cold DMEM0.1%/0.1% buffer and incubated with Alexa Fluor 488econjugated goat anti-mouse secondary antibody (10337882, ThermoFisher) antibody at a 1:200 dilution for 30 min at 4 C in the dark.

    Article Title: Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins.
    Article Snippet: Members of the av family of integrins regulate activation of transforming growth factor beta (TGFb) and are directly involved in pro-tumorigenic phenotypes.. Thus, av integrins may be therapeutic targets for fibrosis and cancer, yet the isolation of selective inhibitors is currently a challenge.. We generated synthetic antibodies selective for av integrins by phage display selections on cell lines that displayed integrin heterodimers.

    Infection:

    Article Title: The Ability of Integrin α v β 3 To Function as a Receptor for Foot-and-Mouth Disease Virus Is Not Dependent on the Presence of Complete Subunit Cytoplasmic Domains
    Article Snippet: Thirty minutes prior to infection, paired transfected cell cultures were incubated at room temperature with the following function-blocking anti-integrin MAbs at a concentration of 25 mg/ml (all antibodies were from Chemicon International Inc): anti-avb3 (clone LM609; MAB1976), anti-avb5 (clone P1F6; MAB1961), anti-avb6 (clone 10D5; MAB2077Z), anti-a5 (clone CLB-705; MAB1986), and anti-b1 (clone 6S6; MAB2253).

    Article Title: Integrin-αvβ6, a Putative Receptor for Foot-and-Mouth Disease Virus, Is Constitutively Expressed in Ruminant Airways
    Article Snippet: Sections were then washed with staining buffer (pH 7.4, PBS supplemented with 4 mM MgCl2 and 0.5% Tween 80) (Sigma-Aldrich), blocked for 15 min with staining buffer 1 10% heat-inactivated normal sheep serum (NShS) and incubated for 1 hr with mouse IgG2a anti-integrin-avb6 (clone 10D5; Chemicon Europe, Chandlers Ford, UK) or control mouse IgG2a (clone OX34; Serotec, Kidlington, UK) diluted to 0.5 mg/ml in staining buffer 1 10% NShS.

    Article Title: Integrin-αvβ6 targeted peptide-toxin therapy in a novel αvβ6-expressing immunocompetent model of pancreatic cancer.
    Article Snippet: Cells were incubated with 1 ml of anti- avb6 antibody (clone 10D5) (MAB2077Z, Millipore) for 1 h at 4 C. The cells werewashed againwith cold DMEM0.1%/0.1% buffer and incubated with Alexa Fluor 488econjugated goat anti-mouse secondary antibody (10337882, ThermoFisher) antibody at a 1:200 dilution for 30 min at 4 C in the dark.

    Article Title: Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins.
    Article Snippet: Members of the av family of integrins regulate activation of transforming growth factor beta (TGFb) and are directly involved in pro-tumorigenic phenotypes.. Thus, av integrins may be therapeutic targets for fibrosis and cancer, yet the isolation of selective inhibitors is currently a challenge.. We generated synthetic antibodies selective for av integrins by phage display selections on cell lines that displayed integrin heterodimers.

    Transfection:

    Article Title: The Ability of Integrin α v β 3 To Function as a Receptor for Foot-and-Mouth Disease Virus Is Not Dependent on the Presence of Complete Subunit Cytoplasmic Domains
    Article Snippet: Thirty minutes prior to infection, paired transfected cell cultures were incubated at room temperature with the following function-blocking anti-integrin MAbs at a concentration of 25 mg/ml (all antibodies were from Chemicon International Inc): anti-avb3 (clone LM609; MAB1976), anti-avb5 (clone P1F6; MAB1961), anti-avb6 (clone 10D5; MAB2077Z), anti-a5 (clone CLB-705; MAB1986), and anti-b1 (clone 6S6; MAB2253).

    Article Title: Integrin-αvβ6, a Putative Receptor for Foot-and-Mouth Disease Virus, Is Constitutively Expressed in Ruminant Airways
    Article Snippet: Sections were then washed with staining buffer (pH 7.4, PBS supplemented with 4 mM MgCl2 and 0.5% Tween 80) (Sigma-Aldrich), blocked for 15 min with staining buffer 1 10% heat-inactivated normal sheep serum (NShS) and incubated for 1 hr with mouse IgG2a anti-integrin-avb6 (clone 10D5; Chemicon Europe, Chandlers Ford, UK) or control mouse IgG2a (clone OX34; Serotec, Kidlington, UK) diluted to 0.5 mg/ml in staining buffer 1 10% NShS.

    Article Title: Integrin-αvβ6 targeted peptide-toxin therapy in a novel αvβ6-expressing immunocompetent model of pancreatic cancer.
    Article Snippet: Cells were incubated with 1 ml of anti- avb6 antibody (clone 10D5) (MAB2077Z, Millipore) for 1 h at 4 C. The cells werewashed againwith cold DMEM0.1%/0.1% buffer and incubated with Alexa Fluor 488econjugated goat anti-mouse secondary antibody (10337882, ThermoFisher) antibody at a 1:200 dilution for 30 min at 4 C in the dark.

    Article Title: Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins.
    Article Snippet: Members of the av family of integrins regulate activation of transforming growth factor beta (TGFb) and are directly involved in pro-tumorigenic phenotypes.. Thus, av integrins may be therapeutic targets for fibrosis and cancer, yet the isolation of selective inhibitors is currently a challenge.. We generated synthetic antibodies selective for av integrins by phage display selections on cell lines that displayed integrin heterodimers.

    Concentration Assay:

    Article Title: The Ability of Integrin α v β 3 To Function as a Receptor for Foot-and-Mouth Disease Virus Is Not Dependent on the Presence of Complete Subunit Cytoplasmic Domains
    Article Snippet: Thirty minutes prior to infection, paired transfected cell cultures were incubated at room temperature with the following function-blocking anti-integrin MAbs at a concentration of 25 mg/ml (all antibodies were from Chemicon International Inc): anti-avb3 (clone LM609; MAB1976), anti-avb5 (clone P1F6; MAB1961), anti-avb6 (clone 10D5; MAB2077Z), anti-a5 (clone CLB-705; MAB1986), and anti-b1 (clone 6S6; MAB2253).

    Article Title: Integrin-αvβ6, a Putative Receptor for Foot-and-Mouth Disease Virus, Is Constitutively Expressed in Ruminant Airways
    Article Snippet: Sections were then washed with staining buffer (pH 7.4, PBS supplemented with 4 mM MgCl2 and 0.5% Tween 80) (Sigma-Aldrich), blocked for 15 min with staining buffer 1 10% heat-inactivated normal sheep serum (NShS) and incubated for 1 hr with mouse IgG2a anti-integrin-avb6 (clone 10D5; Chemicon Europe, Chandlers Ford, UK) or control mouse IgG2a (clone OX34; Serotec, Kidlington, UK) diluted to 0.5 mg/ml in staining buffer 1 10% NShS.

    Article Title: Integrin-αvβ6 targeted peptide-toxin therapy in a novel αvβ6-expressing immunocompetent model of pancreatic cancer.
    Article Snippet: Cells were incubated with 1 ml of anti- avb6 antibody (clone 10D5) (MAB2077Z, Millipore) for 1 h at 4 C. The cells werewashed againwith cold DMEM0.1%/0.1% buffer and incubated with Alexa Fluor 488econjugated goat anti-mouse secondary antibody (10337882, ThermoFisher) antibody at a 1:200 dilution for 30 min at 4 C in the dark.

    Article Title: Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins.
    Article Snippet: Members of the av family of integrins regulate activation of transforming growth factor beta (TGFb) and are directly involved in pro-tumorigenic phenotypes.. Thus, av integrins may be therapeutic targets for fibrosis and cancer, yet the isolation of selective inhibitors is currently a challenge.. We generated synthetic antibodies selective for av integrins by phage display selections on cell lines that displayed integrin heterodimers.



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    90
    Millipore anti- avb6 antibody (clone 10d5)
    Anti Avb6 Antibody (Clone 10d5), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-+avb6+antibody+(clone+10d5)/integrin+avb6+antibody/pm38519394-42-7-13
    Average 90 stars, based on 1 article reviews
    anti- avb6 antibody (clone 10d5) - by Bioz Stars, 2026-10
    90/100 stars
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